Advancements in high-throughput "Omics" techniques have revolutionized plant molecular biology research. Proteomics offers one of the best options for the functional analysis of translated regions of the genome, generating a wealth of detailed information regarding the intrinsic mechanisms of plant stress responses. Various proteomic approaches are being exploited extensively for elucidating master regulator proteins which play key roles in stress perception and signaling, and these approaches largely involve gel-based and gel-free techniques, including both label-based and label-free protein quantification. Furthermore, post-translational modifications, subcellular localization, and protein-protein interactions provide deeper insight into protein molecular function. Their diverse applications contribute to the revelation of new insights into plant molecular responses to various biotic and abiotic stressors.
Subjects
14-3-3 proteins targeted two-dimensional electrophoresis somatic embryogenesis nitrogen metabolism subtilase Sporisorium scitamineum non-orthodox seed antioxidant activity sweet potato plants infected by SPFMV photosynthesis B. acuminata petals chlorophyll deficiency seed proteomics imbibition pollination Sarpo Mira qRT-PCR holm oak tuber phosphoproteome isobaric tags for relative and absolute quantitation (iTRAQ) Quercus ilex nucleotide pyrophosphatase/phosphodiesterase lettuce ?-subunit protein phosphatase germination drought stress pyruvate biosynthesis weakening of carbon metabolism differential proteins heterotrimeric G protein organ LC-MS-based proteomics potato proteomics smut gel-free/label-free proteomics ? subunit shotgun proteomics 2D chloroplast proteome functional annotation Phalaenopsis Clematis terniflora DC wheat Dn1-1 carbon metabolism physiological responses Zea mays phenylpropanoid biosynthesis ISR mass spectrometric analysis patatin leaf pea (Pisum sativum L.) maize ergosterol Camellia sinensis seed storage proteins silver nanoparticles elevated CO2 metacaspase SPV2 and SPVG SnRK1 MALDI-TOF/TOF (phospho)-proteomics leaf spot rice isogenic line wheat leaf rust pathway analysis phosphoproteome sugarcane senescence Oryza sativa L Arabidopsis thaliana heat stress gene ontology innate immunity Pseudomonas syringae bolting chlorophylls shoot Simmondsia chinensis RT-qPCR stresses responses Solanum tuberosum seeds GC-TOF-MS sucrose proteome Puccinia recondita cultivar Zea mays L secondary metabolism ROS Ricinus communis L after-ripening cadmium Stagonospora nodorum virus induced gene silencing quantitative proteomics sweet potato plants non-infected by SPFMV affinity chromatography population variability GS3 fungal perception ammonium transcriptome profiling mass spectrometry analysis papain-like cysteine protease (PLCP) cold stress nitrate late blight disease early and late disease stages seed imbibition lesion mimic mutant protease proteome map seed dormancy petal 2-DE proteomics 2D DIGE root Phytophthora infestans differentially abundant proteins (DAPs) polyphenol oxidase degradome flavonoid 14-3-3 caspase-like proteomics RGG4 co-infection plasma membrane chlorotic mutation Medicago sativa RGG3 glycolysis barley 2-DE protein phosphorylation western blotting N utilization efficiency rice plant pathogenesis responses high temperature data-independent acquisition pattern recognition receptors vegetative storage proteins leaf cell wall proteome plant-derived smoke iTRAQ starch proteome profiling Morus